Georgia Southern Botanical Garden is located a few blocks from the main campus of Georgia Southern University at 1505 Bland Avenue.

From Savannah, take I-16 West to exit 127/Georgia Hwy 67. Take Hwy 67 North (Fair Road) to Statesboro. Turn left on Georgia Avenue, just after the traffic light at East Georgia Regional Hospital. Turn right on Bland Avenue. The Garden gates will be on your right.

From Atlanta, take I-16 east to exit 116/US Hwy 301/25. Take hwy 301 North to Statesboro. Turn right on 301 bypass. Turn left at the first traffic light on Lanier Road. At the dead end, take a right on Georgia Avenue and an immediate left on Bland Avenue. The Garden gates will be on your right.

From Augusta, take US Hwy 25 South to Statesboro. Turn right on Georgia Hwy 67 South. (Note: Hwy 67 South will turn slightly to the left in downtown Statesboro and become Hwy 67 South/Fair Road.) Turn right on Georgia Avenue (the first right after the traffic light at West Gentilly Road). Turn right on Bland Avenue. The Garden gates will be on your right.